Fig. 5. SM compounds degrade HIF-1α through the proteasome using PHDs. a) HCT116 cells were treated with 25 µM 17-AAG or SM122 and/or 1 mM DMOG, which inhibits the PHD prolyl hydroxylases, for 6 hrs in hypoxia. b) Densitometry of nuclear HIF-1α protein levels from three independent experiments. Mean ± SEM.